Journal: bioRxiv

Article Title: Biomimetic, smart and multivalent ligands for G-quadruplex isolation and bioorthogonal imaging

doi: 10.1101/2020.12.18.422878

Figure Lengend Snippet: Structures of the DOTA-based PNA DOTASQ and BioTASQ and the cyclen-based CyTASQ and BioCyTASQ.

Article Snippet: By doing so, we have gradually modified the chemical nature of TASQ from the first biomimetic G4-ligand ( DOTASQ , , and PorphySQ ) to the first smart G4-ligands PNA DOTASQ , ( ) and PNA PorphySQ , whose closed conformation is triggered only by interaction with G4s), twice-as-smart G4-ligands PyroTASQ , and N-TASQ , , smart ligands and smart probes, used to detect G4s in vitro and in cella , and the multivalent G4-ligand BioTASQ , , used as molecular bait for isolating G4 from human cells and identifying them by sequencing.

Techniques:

Journal: bioRxiv

Article Title: Biomimetic, smart and multivalent ligands for G-quadruplex isolation and bioorthogonal imaging

doi: 10.1101/2020.12.18.422878

Figure Lengend Snippet: A . Schematic representation of the FRET-melting assay (upper panel) and results collected with doubly labelled G4s (DNA: F-21-T, F-Myc-T; RNA: F-VEGF-T, F-TERRA-T) and a duplex as a control (F-duplex-T) in the presence of TASQ ( PNA DOTASQ in black, CyTASQ in brown, BioTASQ in gray and BioCyTASQ in red). B . Schematic representation of possible intramolecular H-bond between the biotin appendage and the amide linkage of both PNA DOTASQ and BioTASQ arms. C . Competitive FRET-melting experiments performed with doubly labelled G4-DNA (F-Myc-T) or G4-RNA (F-TERRA-T) in presence of TASQ (CyTASQ in brown, BioCyTASQ in red) and increasing amounts of unlabelled duplex-DNA competitor (ds17, 15 and 50 molar equiv.).

Article Snippet: By doing so, we have gradually modified the chemical nature of TASQ from the first biomimetic G4-ligand ( DOTASQ , , and PorphySQ ) to the first smart G4-ligands PNA DOTASQ , ( ) and PNA PorphySQ , whose closed conformation is triggered only by interaction with G4s), twice-as-smart G4-ligands PyroTASQ , and N-TASQ , , smart ligands and smart probes, used to detect G4s in vitro and in cella , and the multivalent G4-ligand BioTASQ , , used as molecular bait for isolating G4 from human cells and identifying them by sequencing.

Techniques:

Journal: bioRxiv

Article Title: Biomimetic, smart and multivalent ligands for G-quadruplex isolation and bioorthogonal imaging

doi: 10.1101/2020.12.18.422878

Figure Lengend Snippet: A . Schematic representation ( A ) and results ( B ) of the in vitro G4 pull-down protocol performed with FAM-labelled oligonucleotides (the G4s F-Myc, F-22AG and F-SRC; the hairpin F-duplex) and either BioTASQ or BioCyTASQ , quantified by either the decrease of the fluorescence of the supernatant (step 1, C ) or the increase of the fluorescence during the elution (step 2, D ). Competitive pull-down experiments ( E ) performed with F-Myc, F-22AG and F-SRC, Bio ( Cy ) TASQ and the unlabelled duplex competitor ds12 (20 mol. equiv.).

Article Snippet: By doing so, we have gradually modified the chemical nature of TASQ from the first biomimetic G4-ligand ( DOTASQ , , and PorphySQ ) to the first smart G4-ligands PNA DOTASQ , ( ) and PNA PorphySQ , whose closed conformation is triggered only by interaction with G4s), twice-as-smart G4-ligands PyroTASQ , and N-TASQ , , smart ligands and smart probes, used to detect G4s in vitro and in cella , and the multivalent G4-ligand BioTASQ , , used as molecular bait for isolating G4 from human cells and identifying them by sequencing.

Techniques: In Vitro, Fluorescence